Comparative proteome analysis of rubber latex serum from pathogenic fungi tolerant and susceptible rubber tree (Hevea brasiliensis).
Identifieur interne : 000C86 ( Main/Exploration ); précédent : 000C85; suivant : 000C87Comparative proteome analysis of rubber latex serum from pathogenic fungi tolerant and susceptible rubber tree (Hevea brasiliensis).
Auteurs : Phattara-Orn Havanapan [Thaïlande] ; Apichai Bourchookarn [Thaïlande] ; Albert J. Ketterman [Thaïlande] ; Chartchai Krittanai [Thaïlande]Source :
- Journal of proteomics [ 1876-7737 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Hevea, Maladies des plantes.
- métabolisme : Latex, Protéines végétales, Protéome.
- pathogénicité : Phytophthora.
- physiologie : Hevea, Phytophthora.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Latex, Plant Proteins, Proteome.
- microbiology : Hevea, Plant Diseases.
- pathogenicity : Phytophthora.
- physiology : Hevea, Phytophthora.
Abstract
UNLABELLED
Many cultivated rubber trees (Hevea brasiliensis) are invaded by various Phytophthora species fungi, especially in tropical regions which result in crop yield losses. Comparative proteome analysis coupled with liquid chromatography electrospray/ionization (LC-ESI) mass spectrometry identification was employed to investigate the relative abundance of defense related proteins in Phytophthora sp. susceptible (RRIM600) and tolerant (BPM24) clones of rubber tree. Proteome maps of non-rubber constituent of these two model clones show similar protein counts, although some proteins show significant alterations in their abundance. Most of the differentially abundant proteins found in the serum of BPM24 illustrate the accumulation of defense related proteins that participate in plant defense mechanisms such as beta-1,3-glucanase, chitinase, and lectin. SDS-PAGE and 2-D Western blot analysis showed greater level of accumulation of beta-1,3-glucanase and chitinase in latex serum of BPM24 when compared to RRIM600. A functional study of these two enzymes showed that BPM24 serum had greater beta-1,3-glucanase and chitinase activities than that of RRIM600. These up-regulated proteins are constitutively expressed and would serve to protect the rubber tree BPM24 from any fungal invader. The information obtained from this work is valuable for understanding of defense mechanisms and plantation improvement of H. brasiliensis.
BIOLOGICAL SIGNIFICANCE
Non-rubber constituents (latex serum) have almost no value and are treated as waste in the rubber agricultural industry. However, the serum of natural rubber latex contains biochemical substances. The comparative proteomics analysis of latex serum between tolerant and susceptible clones reveals that the tolerant BPM24 clone contained a high abundance of several classes of fungal pathogen-responsive proteins, such as glucanase and chitinase. Moreover, other proteins identified highlighted the accumulation of defensive-associated proteins participating in plant fungal immunity. The isolation of beta-1,3-glucanase, chitinase, and lectin from latex serum should be further investigated and may provide a therapeutic application. This investigation will lead to possible use of latex serum as a great biotechnological resource due to the large quantity of serum produced and the biochemicals contained therein.
DOI: 10.1016/j.jprot.2015.10.014
PubMed: 26477389
Affiliations:
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Electronic address: phattaraorn.hav@mahidol.ac.th.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom</wicri:regionArea><wicri:noRegion>Nakhonpathom</wicri:noRegion></affiliation></author><author><name sortKey="Bourchookarn, Apichai" sort="Bourchookarn, Apichai" uniqKey="Bourchookarn A" first="Apichai" last="Bourchookarn">Apichai Bourchookarn</name><affiliation wicri:level="1"><nlm:affiliation>Department of Technology and Industries, Faculty of Science and Technology, Prince of Songkla University, Pattani, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Technology and Industries, Faculty of Science and Technology, Prince of Songkla University, Pattani</wicri:regionArea><wicri:noRegion>Pattani</wicri:noRegion></affiliation></author><author><name sortKey="Ketterman, Albert J" sort="Ketterman, Albert J" uniqKey="Ketterman A" first="Albert J" last="Ketterman">Albert J. Ketterman</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom</wicri:regionArea><wicri:noRegion>Nakhonpathom</wicri:noRegion></affiliation></author><author><name sortKey="Krittanai, Chartchai" sort="Krittanai, Chartchai" uniqKey="Krittanai C" first="Chartchai" last="Krittanai">Chartchai Krittanai</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom</wicri:regionArea><wicri:noRegion>Nakhonpathom</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of proteomics</title><idno type="eISSN">1876-7737</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Hevea (microbiology)</term><term>Hevea (physiology)</term><term>Latex (metabolism)</term><term>Phytophthora (pathogenicity)</term><term>Phytophthora (physiology)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (metabolism)</term><term>Proteome (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Hevea (microbiologie)</term><term>Hevea (physiologie)</term><term>Latex (métabolisme)</term><term>Maladies des plantes (microbiologie)</term><term>Phytophthora (pathogénicité)</term><term>Phytophthora (physiologie)</term><term>Protéines végétales (métabolisme)</term><term>Protéome (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Latex</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Hevea</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Hevea</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Latex</term><term>Protéines végétales</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Hevea</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Hevea</term><term>Phytophthora</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>UNLABELLED</b></p><p>Many cultivated rubber trees (Hevea brasiliensis) are invaded by various Phytophthora species fungi, especially in tropical regions which result in crop yield losses. Comparative proteome analysis coupled with liquid chromatography electrospray/ionization (LC-ESI) mass spectrometry identification was employed to investigate the relative abundance of defense related proteins in Phytophthora sp. susceptible (RRIM600) and tolerant (BPM24) clones of rubber tree. Proteome maps of non-rubber constituent of these two model clones show similar protein counts, although some proteins show significant alterations in their abundance. Most of the differentially abundant proteins found in the serum of BPM24 illustrate the accumulation of defense related proteins that participate in plant defense mechanisms such as beta-1,3-glucanase, chitinase, and lectin. SDS-PAGE and 2-D Western blot analysis showed greater level of accumulation of beta-1,3-glucanase and chitinase in latex serum of BPM24 when compared to RRIM600. A functional study of these two enzymes showed that BPM24 serum had greater beta-1,3-glucanase and chitinase activities than that of RRIM600. These up-regulated proteins are constitutively expressed and would serve to protect the rubber tree BPM24 from any fungal invader. The information obtained from this work is valuable for understanding of defense mechanisms and plantation improvement of H. brasiliensis.</p></div><div type="abstract" xml:lang="en"><p><b>BIOLOGICAL SIGNIFICANCE</b></p><p>Non-rubber constituents (latex serum) have almost no value and are treated as waste in the rubber agricultural industry. However, the serum of natural rubber latex contains biochemical substances. The comparative proteomics analysis of latex serum between tolerant and susceptible clones reveals that the tolerant BPM24 clone contained a high abundance of several classes of fungal pathogen-responsive proteins, such as glucanase and chitinase. Moreover, other proteins identified highlighted the accumulation of defensive-associated proteins participating in plant fungal immunity. The isolation of beta-1,3-glucanase, chitinase, and lectin from latex serum should be further investigated and may provide a therapeutic application. This investigation will lead to possible use of latex serum as a great biotechnological resource due to the large quantity of serum produced and the biochemicals contained therein.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26477389</PMID><DateCompleted><Year>2016</Year><Month>09</Month><Day>06</Day></DateCompleted><DateRevised><Year>2017</Year><Month>07</Month><Day>29</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1876-7737</ISSN><JournalIssue CitedMedium="Internet"><Volume>131</Volume><PubDate><Year>2016</Year><Month>Jan</Month><Day>10</Day></PubDate></JournalIssue><Title>Journal of proteomics</Title><ISOAbbreviation>J Proteomics</ISOAbbreviation></Journal><ArticleTitle>Comparative proteome analysis of rubber latex serum from pathogenic fungi tolerant and susceptible rubber tree (Hevea brasiliensis).</ArticleTitle><Pagination><MedlinePgn>82-92</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1874-3919(15)30160-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jprot.2015.10.014</ELocationID><Abstract><AbstractText Label="UNLABELLED">Many cultivated rubber trees (Hevea brasiliensis) are invaded by various Phytophthora species fungi, especially in tropical regions which result in crop yield losses. Comparative proteome analysis coupled with liquid chromatography electrospray/ionization (LC-ESI) mass spectrometry identification was employed to investigate the relative abundance of defense related proteins in Phytophthora sp. susceptible (RRIM600) and tolerant (BPM24) clones of rubber tree. Proteome maps of non-rubber constituent of these two model clones show similar protein counts, although some proteins show significant alterations in their abundance. Most of the differentially abundant proteins found in the serum of BPM24 illustrate the accumulation of defense related proteins that participate in plant defense mechanisms such as beta-1,3-glucanase, chitinase, and lectin. SDS-PAGE and 2-D Western blot analysis showed greater level of accumulation of beta-1,3-glucanase and chitinase in latex serum of BPM24 when compared to RRIM600. A functional study of these two enzymes showed that BPM24 serum had greater beta-1,3-glucanase and chitinase activities than that of RRIM600. These up-regulated proteins are constitutively expressed and would serve to protect the rubber tree BPM24 from any fungal invader. The information obtained from this work is valuable for understanding of defense mechanisms and plantation improvement of H. brasiliensis.</AbstractText><AbstractText Label="BIOLOGICAL SIGNIFICANCE" NlmCategory="UNASSIGNED">Non-rubber constituents (latex serum) have almost no value and are treated as waste in the rubber agricultural industry. However, the serum of natural rubber latex contains biochemical substances. The comparative proteomics analysis of latex serum between tolerant and susceptible clones reveals that the tolerant BPM24 clone contained a high abundance of several classes of fungal pathogen-responsive proteins, such as glucanase and chitinase. Moreover, other proteins identified highlighted the accumulation of defensive-associated proteins participating in plant fungal immunity. The isolation of beta-1,3-glucanase, chitinase, and lectin from latex serum should be further investigated and may provide a therapeutic application. This investigation will lead to possible use of latex serum as a great biotechnological resource due to the large quantity of serum produced and the biochemicals contained therein.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Havanapan</LastName><ForeName>Phattara-Orn</ForeName><Initials>PO</Initials><AffiliationInfo><Affiliation>Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand. Electronic address: phattaraorn.hav@mahidol.ac.th.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bourchookarn</LastName><ForeName>Apichai</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Technology and Industries, Faculty of Science and Technology, Prince of Songkla University, Pattani, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ketterman</LastName><ForeName>Albert J</ForeName><Initials>AJ</Initials><AffiliationInfo><Affiliation>Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Krittanai</LastName><ForeName>Chartchai</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>10</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Proteomics</MedlineTA><NlmUniqueID>101475056</NlmUniqueID><ISSNLinking>1874-3919</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007840">Latex</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D028482" MajorTopicYN="N">Hevea</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007840" MajorTopicYN="N">Latex</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Comparative proteomics</Keyword><Keyword MajorTopicYN="N">Hevea brasiliensis</Keyword><Keyword MajorTopicYN="N">Latex serum</Keyword><Keyword MajorTopicYN="N">Phytophthora sp.</Keyword><Keyword MajorTopicYN="N">Rubber tree</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>08</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>09</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>10</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26477389</ArticleId><ArticleId IdType="pii">S1874-3919(15)30160-3</ArticleId><ArticleId IdType="doi">10.1016/j.jprot.2015.10.014</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Thaïlande</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Havanapan, Phattara Orn" sort="Havanapan, Phattara Orn" uniqKey="Havanapan P" first="Phattara-Orn" last="Havanapan">Phattara-Orn Havanapan</name></noRegion><name sortKey="Bourchookarn, Apichai" sort="Bourchookarn, Apichai" uniqKey="Bourchookarn A" first="Apichai" last="Bourchookarn">Apichai Bourchookarn</name><name sortKey="Ketterman, Albert J" sort="Ketterman, Albert J" uniqKey="Ketterman A" first="Albert J" last="Ketterman">Albert J. Ketterman</name><name sortKey="Krittanai, Chartchai" sort="Krittanai, Chartchai" uniqKey="Krittanai C" first="Chartchai" last="Krittanai">Chartchai Krittanai</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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